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low  (New England Biolabs)


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    Structured Review

    New England Biolabs low
    Low, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/molecular+weight+dna+ladder/Low+Molecular+Weight+DNA+Ladder/pm42881293-198-145-149
    Average 96 stars, based on 795 article reviews
    low - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: A spatially-programmed DNA nanoplatform for multiplexed and orthogonal miRNA profiling enables robust diagnosis of colorectal cancer.
    Article Snippet: Sensitive and simultaneous detection of a panel of microRNAs (miRNAs) is crucial for the precise early diagnosis of colorectal cancer (CRC) in complex environments and clinical samples.. Here, we developed a CRC screening platform based on a multi-branched DNA scaffold assembled with multiple fluorophore-modified DNA hairpins for the simultaneous analysis of three CRC-associated miRNAs (miR-31, miR-576, and miR-4669).. The spatiallyprogrammed DNA nanoplatform provided an ideal framework for the precise organization of DNA hairpins at the nanoscale, which function as both target recognition elements and independent catalytic hairpin assembly (CHA) modules.

    Article Title: Reference gene evaluation for digital PCR; Applications for RNA biomarker testing in cervical precancer.
    Article Snippet: .. Samples were run on a 12 % acrylamide gel at 100 V for 2 h. A low molecular weight DNA ladder (New England Biolabs) was included to allow accurate sizing of PCR products. ..

    Article Title: Peptides and compounds that bind to elongation initiation factor 4E
    Article Snippet: Briefly, the library DNA (15 samples, 50 ng each) was amplified in a total volume of 25 μL with 1× Phusion® buffer, 200 μM each dNTPs, 1.5 mM MgCl2, 0.5 μM forward primer, 0.5 μM reverse barcoded primer, and one unit Phusion® High-Fidelity DNA Polymerase. .. PCR was performed using the following thermo cycler program: 95° C. for 30 s, followed by 25 cycles of 95° C. for 10 s, 60.5° C. for 15 s and 72° C. for 30 s, and then a final extension at 72° C. for 5 min before holding at 4° C. The dsDNA fragments from the PCR were quantified by running at 2% (w/v) agarose gel in Tris-Borate-EDTA buffer at 100 volts for ̃45 min using a low molecular weight DNA ladder as a standard (NEB, #N3233S). .. The dsDNA fragments (15 samples, 40 ng per sample) were pooled together and purified on E-Gel® SizeSelectTM 2% agarose gel (Invitrogen, #G6610-02).

    Article Title: Target-Induced Aggregation of 3D DNA Lipidic Nanoparticles via Catalytic Hairpin Assembly Reactions for Enhanced Imaging of Endogenous miRNA
    Article Snippet: Nucleic acid probes, while promising for intracellular biomarker detection, are limited by poor biostability, slow kinetics, and weak signal amplification in live cells.. To overcome these challenges, we present a catalytic hairpin assembly (CHA)-driven strategy that enables target-induced aggregation of DNA-functionalized lipidic nanoparticles (TIA-LNPs) for highly sensitive imaging of endogenous microRNA.. The system employs spherical lipidic nanoparticles self-assembled from cholesterol-modified DNA strands, which are further equipped with DNA hairpin structures to facilitate cellular uptake and maintain intracellular integrity.

    Article Title: Distinct spatial organisation of Rho and RNA Polymerase in Salmonella cells
    Article Snippet: .. The lengths of minor termination products (150-300 nt range) were estimated from gel migration using the molecular size calculation function of ImageQuant TL (GE Healthcare), calibrated with a low molecular weight DNA ladder (New England Biolabs). ..

    Article Title: Highly efficient CRISPR-mediated genome editing through microfluidic droplet cell mechanoporation
    Article Snippet: .. DNA sample was loaded together with low molecular weight DNA ladder (NEB N3233S). .. For HDR analysis using restriction enzyme digestion, the 200 ng purified PCR product was incubated with 5 units of EcoRV-HF(NEB) in rCutSmart buffer at 37 °C.

    Article Title: Evaluation of multiplexed liquid glycan Array (LiGA) for serological detection of glycan-binding antibodies
    Article Snippet: .. The PCR products were quantified by 2% (w/v) agarose gel in Tris-Borate-EDTA buffer at 100 volts for ~35 min using a low molecular weight DNA ladder as standard (NEB, #N3233S). ..

    Acrylamide Gel Assay:

    Article Title: Reference gene evaluation for digital PCR; Applications for RNA biomarker testing in cervical precancer.
    Article Snippet: .. Samples were run on a 12 % acrylamide gel at 100 V for 2 h. A low molecular weight DNA ladder (New England Biolabs) was included to allow accurate sizing of PCR products. ..

    Polymerase Chain Reaction:

    Article Title: Reference gene evaluation for digital PCR; Applications for RNA biomarker testing in cervical precancer.
    Article Snippet: .. Samples were run on a 12 % acrylamide gel at 100 V for 2 h. A low molecular weight DNA ladder (New England Biolabs) was included to allow accurate sizing of PCR products. ..

    Article Title: Peptides and compounds that bind to elongation initiation factor 4E
    Article Snippet: Briefly, the library DNA (15 samples, 50 ng each) was amplified in a total volume of 25 μL with 1× Phusion® buffer, 200 μM each dNTPs, 1.5 mM MgCl2, 0.5 μM forward primer, 0.5 μM reverse barcoded primer, and one unit Phusion® High-Fidelity DNA Polymerase. .. PCR was performed using the following thermo cycler program: 95° C. for 30 s, followed by 25 cycles of 95° C. for 10 s, 60.5° C. for 15 s and 72° C. for 30 s, and then a final extension at 72° C. for 5 min before holding at 4° C. The dsDNA fragments from the PCR were quantified by running at 2% (w/v) agarose gel in Tris-Borate-EDTA buffer at 100 volts for ̃45 min using a low molecular weight DNA ladder as a standard (NEB, #N3233S). .. The dsDNA fragments (15 samples, 40 ng per sample) were pooled together and purified on E-Gel® SizeSelectTM 2% agarose gel (Invitrogen, #G6610-02).

    Article Title: Evaluation of multiplexed liquid glycan Array (LiGA) for serological detection of glycan-binding antibodies
    Article Snippet: .. The PCR products were quantified by 2% (w/v) agarose gel in Tris-Borate-EDTA buffer at 100 volts for ~35 min using a low molecular weight DNA ladder as standard (NEB, #N3233S). ..

    Agarose Gel Electrophoresis:

    Article Title: Peptides and compounds that bind to elongation initiation factor 4E
    Article Snippet: Briefly, the library DNA (15 samples, 50 ng each) was amplified in a total volume of 25 μL with 1× Phusion® buffer, 200 μM each dNTPs, 1.5 mM MgCl2, 0.5 μM forward primer, 0.5 μM reverse barcoded primer, and one unit Phusion® High-Fidelity DNA Polymerase. .. PCR was performed using the following thermo cycler program: 95° C. for 30 s, followed by 25 cycles of 95° C. for 10 s, 60.5° C. for 15 s and 72° C. for 30 s, and then a final extension at 72° C. for 5 min before holding at 4° C. The dsDNA fragments from the PCR were quantified by running at 2% (w/v) agarose gel in Tris-Borate-EDTA buffer at 100 volts for ̃45 min using a low molecular weight DNA ladder as a standard (NEB, #N3233S). .. The dsDNA fragments (15 samples, 40 ng per sample) were pooled together and purified on E-Gel® SizeSelectTM 2% agarose gel (Invitrogen, #G6610-02).

    Article Title: Evaluation of multiplexed liquid glycan Array (LiGA) for serological detection of glycan-binding antibodies
    Article Snippet: .. The PCR products were quantified by 2% (w/v) agarose gel in Tris-Borate-EDTA buffer at 100 volts for ~35 min using a low molecular weight DNA ladder as standard (NEB, #N3233S). ..

    Migration:

    Article Title: Distinct spatial organisation of Rho and RNA Polymerase in Salmonella cells
    Article Snippet: .. The lengths of minor termination products (150-300 nt range) were estimated from gel migration using the molecular size calculation function of ImageQuant TL (GE Healthcare), calibrated with a low molecular weight DNA ladder (New England Biolabs). ..



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    Image Search Results


    Preparation Scale and Cost Estimates for In-House and Commercial Nucleic Acid Ladders

    Journal: ACS Omega

    Article Title: The Dual-Ribozyme RNA Ladder and RNA-Calibrated ssDNA Marker System for Inexpensive RNA Size Standards

    doi: 10.1021/acsomega.6c08063

    Figure Lengend Snippet: Preparation Scale and Cost Estimates for In-House and Commercial Nucleic Acid Ladders

    Article Snippet: NEB Low Molecular Weight DNA Ladder , N/A , $73.53 , 50 , $1.47 , $147.06.

    Techniques: Molecular Weight, Marker